over-express functionally active proteins
The Structural Genomics Consortium (SGC) has produced a unique resource of verified expression clones, which can be used to generate purified, active proteins for multiple uses, including crystallization, biochemical assays, ligand/drug screening and antibody generation. Target proteins have been chosen based on medical relevance, feasibility and scientific impact. The focus thus far has been on human proteins of biomedical importance, as well as proteins from human parasites. Each clone in the collection has been verified by the SGC to express the expected protein from the bacterial host however most do not contain the full-length gene.
Protein purification protocols and additional information on each protein can be found on the SGC website:
http://www.thesgc.com/structures/
3D structures for all proteins encoded by the SGC collection are available from the Protein Data Bank (PDB):
http://www.wwpdb.org/
An additional resource provided by the SGC is the “interactive Structurally enhanced experience” (iSee). Detailed descriptions along with interactive graphic representations provide the user with useful and relevant information about their proteins of interest. All levels of the workflow from DNA to protein structure are integrated with expert analysis. Information is contained and delivered in a simple computer file that can be run on either Linux, Windows, or Mac OS X. (Abagyan, R., et al., (2006)).
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| We provide certain clone resources developed by leading academic laboratories. Many of these resources address the needs of specialized research communities not served by other commercial entities. In order to provide these as a public resource, we depend on the contributing academic laboratories for quality control. Therefore, these are distributed in the format provided by the contributing institution "as is" with no additional product validation or guarantee. Thermo Fisher Scientific is not responsible for any errors or performance issues. Additional information can be found in the product manual as well as in associated published articles (if available). Alternatively, the source academic institution can be contacted directly for troubleshooting. |
Individual clones:
Clones are provided as a live culture in a 2-ml tube. Each tube contains LB medium supplemented with 8% glycerol and the appropriate antibiotic. Within 2–3 business days of receiving your order we will ship your clone at room temperature via express delivery. Store the stock clone at –80°C.
Clone Collections:
Orders of 50 clones or greater and stock plates will be provided in 96-well microtiter plates. These will ship on dry-ice via overnight delivery and should be stored at –80°C immediately upon receipt. Please contact our customer service department for a quote and estimated shipping time on bulk orders.
References:
Structural Genomics Consortium, et al., (2008) “Protein production and purification” Nat Methods, Vol 5:2;135–146.
Abagyan, R., et al., (2006) “Disseminating structural genomics data to the public: from a data dump to an animated story” TiBS, Vol 31:2;76-78.
Federov, O., et al., (2007) “A systematic interaction map of validated kinase inhibitors with Ser/Thr kinases” Proc Natl Acad Sci U S A., Vol 104:31;20523-20538.
Pogacic, V., et al., (2007) "Structural analysis identifies imidazo[1,2-b]pyridazines as PIM kinase inhibitors with in vitro antileukemic activity" Cancer Res., Vol 67:14;6916-6924.
