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Negative Selection Screens

pGIPZ
Vector Element
Utility
CMV Promoter
RNA Polymerase II promoter
cPPT
Central Polypurine tract
helps translocation into the nucleus of non-dividing cells
WRE
Enhances the stability and translation of transcripts
turbo GFP
Marker to track shRNAmir expression
Puror
Mammalian selectable marker
AMPr
Ampicillin bacterial selectable marker
5'LTR
5' long terminal repeat
pUC ori
High copy replication and maintenance in e.coli
SIN-LTR
3' Self inactivating long terminal repeat
RRE
Rev response element
ZEOr
Bacterial selectable marker
 
Purchasing Information
Cat # Description Literature ACA COM Buy
RHS4430
Human GIPZ lentiviral shRNAmir individual clone   $209.00* $209.00* Click to show search field
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(only clones from RHS4430)
RMM4431 Mouse GIPZ lentiviral shRNAmir individual clone   $209.00* $209.00* Click to show search field
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(only clones from RMM4431)
VGH5518
Human GIPZ lentiviral shRNAmir individual clone -viral particles   $765.00* $765.00* Click to show search field
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(only clones from VGH5518)
VGM5520 Mouse GIPZ lentiviral shRNAmir individual clone - viral particles   $765.00* $765.00* Click to show search field
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(only clones from VGM5520)
RHS4287
RNAintro GIPZ lentiviral shRNAmir transfection starter kit   $902.00* $1,351.00* Click to assemble kit
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(only clones from RHS4287)
RHS4346 Non-silencing-GIPZ lentiviral shRNAmir control-Glycerol stock   $428.00* $428.00*
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(only clones from RHS4346)
RHS4349
pGIPZ lentiviral empty vector - Glycerol   $321.00* $321.00*
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(only clones from RHS4349)
RHS4371 GAPDH-GIPZ lentiviral shRNAmir positive control-Glycerol stock   $428.00* $428.00*
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(only clones from RHS4371)
RHS4480
EG5-GIPZ lentiviral shRNAmir positive control-Glycerol stock   $428.00* $428.00*
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(only clones from RHS4480)
RHS5828 Human GIPZ lentiviral shRNAmir library subscription (Releases 6.1-6.18)   call call Click to show search field
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(only clones from RHS5828)
RMM5829
Mouse GIPZ lentiviral shRNAmir library subscription (Releases 7.1-7.17)   call call Click to show search field
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(only clones from RMM5829)
RHS4828 Validated GIPZ lentiviral shRNAmir human cancer gene set   call call
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(only clones from RHS4828)
RHS5086
RNAintro GIPZ lentiviral shRNAmir transduction kit   $1,341.00* $1,759.00* Click to assemble kit
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(only clones from RHS5086)
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Thermo Scientific Open Biosystems
GIPZ Lentiviral shRNAmir

shRNAmir-GFP: Visualize RNAi
  • shRNAmir design for specificity and increased knockdown efficiency
  • TurboGFP marks cells expressing shRNAmir
  • Efficient low copy knockdown- Important for pooled screens
  • Genome-wide human and mouse coverage
  • Perform efficient RNAi in primary and non-dividing cells
  • Guaranteed* knockdown

Please note that the pGIPZ and pTRIPZ vectors are not compatible with third generation packaging systems such as ViraPower™ from Invitrogen. We recommend the Trans-Lentiviral™ Packaging System for use with our vectors.

Transduction efficiency of pGIPZ
Transduction Efficiency
TurboGFP expression from the pGIPZ vector allows measurment of transfection and transduction efficiency. It also effectively marks shRNAmir expression in target cells. A. HEK293 cells 48 hrs post-transduction and B. the corrosponding phase image. 100% transduction efficiency is seen in these cells

Knockdown efficiency of GIPZ lentiviral shRNAmir


Knockdown Efficiency
HEK293T cells were transduced with lentiviral particles containing shRNAmir to GAPDH, eG5, p53, Rb or STAT6. non-silencing shRNAmir was transduced as a control. The graph depicts the residual levels of these genes normalized to their equivalent non-silencing control
The GIPZ lentiviral shRNAmir library can be purchased as individual human or mouse constructs, target gene sets, RNAintro starter kits or entire libraries. Validated GIPZ controls- positive and negative controls are available in glycerol stock or viral particle formats.

High titer lentiviral particles are now available for GIPZ individual constructs
Industry-leading high-titer purified virus is now offered at 1x108 TU/ml in 2x25 ul aliquots. This allows for multiple experiments in a variety of cell types requiring variable multiplicity of infection (MOI) and minimizes toxicity. Transduction-ready high-titer lentiviral particle format reduces time, labor and costs involved in prepping DNA, packaging and purifying lentivirus for your RNAi experiments.
Purchase 3 constructs per target gene and SAVE over 50%, receive FREE non-silencing and GAPDH positive controls
AND guaranteed** knockdown

Additional GIPZ lentiviral shRNAmir sub-libraries include:
Validated human GIPZ cancer set
GO annotated mouse GIPZ gene families

*When used in GIPZ RNAintro kits according to kit instructions.
 
**One out of three constructs in lentiviral particle format is guaranteed to knockdown at greater than 70% at the mRNA level. Knockdown should be compared to non-silencing control and experimental workflow controlled using the GAPDH positive control (provided).

Finding your shRNAmir of interest

  1. Enter gene symbol(s), GenBank accession number(s), clone IDs, unigene clusters or catalog numbers into the gene search or into the shRNA search () in the purchase grid. Results will be returned in a tabbed format. Click on shRNAs under the shRNAs/RNAi tab.
  2. Target gene sets will be shown for all targets that have greater than two shRNAmir/target. Click on the oligo ID link for additional information including hairpin sequences.
  3. To order viral formats click on the shRNA custom viral production tab and select your construct/s of interest. Select 3 constructs per target gene to save over 50%, get free controls and get guaranteed** knockdown.
  4. Order directly online. Constructs are shipped in 48 hours.
Alternatively you can send your gene symbols or accession numbers to us at info@openbiosystems.com and our technical representatives will perform your search for you.

This product is covered by the following limited use licenses:

Limited Use License-shRNA technologies
Limited Use License-ddRNAi
TurboGFP and TurboRFP are covered by a Limited Use License from Evrogen.
Shipping Information:
The GIPZ lentiviral shRNAmir library is provided in 96-well microtiter plates containing frozen stock cultures of E.coli in LB (low salt) broth with 8% glycerol, carbenicillin (100ug/ml) and zeocin (25ug/ml). Individual shRNAmir constructs are shipped either as glycerol stock cultures on wet ice or as high-titer lentiviral particles on dry ice. Open Biosystems checks all cultures for growth prior to shipment. Store at -80ºC.

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